Affymetrix expression analysis technical manual

Expression Analysis Technical Manual For HT Array Plates Using the GeneChip® Array Station (pdf, MB) Expression Analysis Technical Manual, with Specific Protocols for Use with the Hybridization, Wash, and Stain Kit (pdf, MB) Expression Wash, Stain and Scan User Manual for Cartridge Arrays (pdf, MB). The Affymetrix GeneChip® Expression Analysis Technical Manual is designed for use in a system consisting of a GeneChip® Fluidics Station , a GeneChip® Hybridization Oven and a GeneArray® Scanner. References 1. Sambrook, J., Fritsch, E.F., Maniatis, T. Molecular Cloning: A Laboratory Manual, v in Affymetrix GeneChip Fluidics Station with Euk Ge-WS2v5 fluidics script according to the instructions in the technical manual. Affymetrix GeneChip Scanner was used for scanning the arrays. Data analysis, preparation of gene list, and pathways Preliminary and advanced analysis of the scanned chips was.

included in this manual have been used successfully by scientists at Affymetrix, or have been recommended by our collaborators during the development of particular products. The field of mRNA gene expression monitoring is rapidly evolving and periodic technical updates to this manual will reflect the newest protocols and information for using GeneChip probe arrays. This manual applies to all GeneChip expression products. The Affymetrix GeneChip® Expression Analysis Technical Manual is designed for use in a system consisting of a GeneChip® Fluidics Station , a GeneChip® Hybridization Oven and a GeneArray® Scanner. References 1. Sambrook, J., Fritsch, E.F., Maniatis, T. Molecular Cloning: A Laboratory Manual, v Complete Manual: Expression Analysis Technical Manual (with hyperlinks) (zip, MB) Expression Analysis Technical Manual (for print) (zip, KB) Sections 1 - 4: (Manual Sections) Section 1: GeneChip Expression Analysis Overview (pdf, KB) Section 2: Eukaryotic Sample and Array Processing (pdf, KB).

For the 3' arrays, continue to follow the protocols detailed in the GeneChip® Expression Analysis Technical. Manual (P/N ). Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 1 ug total RNA (Expression Analysis Technical Manual, Affymetrix). 1. Gene Chip® Human Genome U Plus Array (Affymetrix) Affymetrix's recommendations (GeneChip Expression Analysis Technical Manual).